Novel RNA Seq-based know-how gives unbiased profiling of all seven human TCR and BCR variable areas from a single pattern

Cellecta, Inc. right now introduced the launch of the DriverMap™ Adaptive Immune Receptor (AIR) TCR and BCR kits, a household of multiplex-PCR, NGS-based assays that allow profiling of as much as all seven T-cell receptor (TCR) and B-cell receptor (BCR) chains from the identical pattern, in a single tube, with out want of specialised tools.

Ranging from quite a lot of immune pattern kind inputs—complete blood, tissue biopsies, FFPE and extra—the DriverMap AIR kits use functionally validated primer units which goal the extremely variable third complementarity figuring out area (CDR3) of the heavy chain of the respective Tand B-cell receptors, together with reagents for multiplex PCR, to yield NGS-ready libraries. Subsequent sequencing and evaluation establish antigen-activated clonotypes which might present perception into the adaptive immune response and is helpful for a variety of therapeutic and diagnostic purposes.

“Whereas there are quite a few immune receptor profiling choices accessible in the marketplace right now, there are none that present the excellent, extremely delicate and quantifiable outcomes offered by the DriverMap AIR TCR and BCR assays. With this know-how, we’re happy to offer researchers with an environment friendly instrument to help their efforts in discovering and validating novel predictive and prognostic immunity biomarkers,” mentioned Alex Chenchik, Ph.D., president and chief scientific officer of Cellecta.

The extremely reproducible Driver-Map™ AIR assay represents a number of essential enhancements over competitor assays.

• Unbiased, cost-effective profiling of all TCR/BCR mRNA isoforms in a single assay and quantitative immune receptor repertoire protection of considerable clonotypes from complete blood, PBMC, FFPE, tissue or purified immune cells.
• Reproducible, quantitative evaluation of useful, productive, considerable AIR clonotypes utilizing common molecular index (UMI) know-how.
• Particular profiling of AIR repertoire with out background of primer-dimer and offtarget amplification merchandise.

Along with the DriverMap AIR kits, Cellecta continues to offer the DriverMap AIR Profiling assay as a service for collaborators working in quite a lot of therapeutic areas. Researchers ship Cellecta their immune cell samples and obtain quantitative clonotype knowledge and an evaluation report generated by MiXCR software program in just some weeks.